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Proteinase K w/ Storage Buffer Set
Highlights
Lyophilized enzyme provided with Proteinase K Storage Buffer.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Lyophilized enzyme provided with Proteinase K Storage Buffer.

Product Description

Proteinase K is a stable serine protease with broad substrate specificity and will degrade many proteins in their native conformation even in the presence of detergents (e.g., SDS). The enzyme is frequently used in molecular biology applications to digest unwanted proteins such as nucleases from DNA and/or RNA preparations from microorganisms, cells, and plants.

Technical Specifications

Enzyme Commission Number(EC 3.4.21.64)
pH and Temperature Range4.0 to 12.0 (8.0 is optimum), 25 to 65°C
SourceEngyodontium album
Specific Activity> 30 units/mg protein
Unit DefinitionOne unit (U) of enzyme will hydrolyze urea-denatured hemoglobin to produce 1.0 µmole of tyrosine per minute at pH 7.5 at 37°C.

Resources

Q1: What volume of Proteinase K Storage Buffer is needed to reconstitute lyophilized Proteinase K?
SizeAdd Proteinase K Storage Buffer
5 mg260 µl
20 mg1040 µl
60 mg3120 µl
125 mg6500 µl
Reconstitute lyophilized Proteinase K at 20 mg/ml with Proteinase K Storage Buffer. Table provides recommended volumes for different sample sizes (mg); Concentration can be adjusted as needed.

 

주문정보

CAT.No 품명 규격 비고
D3001-2-5 Proteinase K w/ Storage Buffer Set 5 mg Lyophilized enzyme provided w/ storage buffer
D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg Lyophilized enzyme provided w/ storage buffer
D3001-2-60 Proteinase K w/ Storage Buffer Set 60 mg Lyophilized enzyme provided w/ storage buffer
D3001-2-125 Proteinase K w/ Storage Buffer Set 125 mg Lyophilized enzyme provided w/ storage buffer