Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Highest Yield: Robust cfDNA isolation from up to 10 mL plasma w/ unique MAGicBead™ surface technology
NGS-Ready: High-quality cfDNA from plasma, saliva, urine, etc. is ideal for NGS
Compatible: With all open automation platforms
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Highest Yield: Robust cfDNA isolation from up to 10 mL plasma w/ unique MAGicBead™ surface technology
NGS-Ready: High-quality cfDNA from plasma, saliva, urine, etc. is ideal for NGS
Compatible: With all open automation platforms
Product Description
The MAGicBead™ cfDNA Isolation Kit features a unique, magnetic bead surface chemistry that facilitates unparalleled cfDNA recovery from biofluids. Input volumes can range up to 10 mL of plasma. It is compatible with all open automation platforms and a wide range of sample types (serum, saliva, urine, etc.). This kit features a simple, streamlined extraction process with minimal number of steps and does not require the bead drying step common with other similar procedures. Eluted cfDNA is free of enzymatic inhibitors that can interfere with PCR or other sensitive downstream applications, including Next-Gen Sequencing.
Technical Specifications
Elution Volume
≥ 15 µl MAGicBead™ cfDNA Elution Buffer.
Fragment Sizes
For plasma samples, DNA fragments will typically display a mono-nucleosomal peak in the 140-170 bp range and a genomic DNA fragments peak > 10 kb. However, other multi-nucleosomal apoptotic DNA fragments may also be present.
Processing Time
1-1.5 hour (~15 minutes hands-on time).
Purity
Eluted cfDNA is NGS-ready. NOTE: Due to low levels of cfDNA available in cell-free biofluids, a spectrophotometer (e.g., NanoDrop) is not recommended for quantification or purity assessment.
Required Equipment
An open automation platform: KingFisher Flex/Apex (Thermo-Fisher Scientific), Fluent® X (Tecan), Microlab® STAR™ (Hamilton), OT-2 (Opentrons) and others. For manual applications, a magnetic stand compatible with conical/centrifuge tubes/plates and a rotator capable of at least 30 rpm are needed.
Sample Compatibility
Plasma samples derived from most blood collection tube types are compatible, including Streck DNA BCT. Plasma samples derived from blood collection tubes with heparin are NOT compatible. Highly viscous biofluids, such as whole saliva and synovial fluids, may have limited compatibility. Refer to Appendix A (Page 6) for more details.
Yield
Typical yields range from 0.5 ng to 20 ng from healthy donor plasma samples. This can range from 0.1 ng to 100 ng depending on the health status of donors.
No. MAGicBead™ cfDNA will recover gDNA if present in your sample. We recommend applying size select (Zymo Research Select-a-Size DNA Clean and Concentrator MagBead Kit, D4084 or similar.) if your downstream assay is sensitive to presence of gDNA.
Yes, MAGicBead™ cfDNA is also compatible with serum, urine, saliva, cerebrospinal fluid, amniotic fluids, spent cell culture media, pleural fluid, bile acid and bronchoalveolar lavage.
No, we do not recommend quantifying using spectrophotometers as a minimum of 10 ng/µL is required for a reliable quantification and purity assessment, which cell-free biofluids rarely yield.
