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Zymo Research
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ZR BAC DNA Miniprep Kit
Highlights

High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.

Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization

Unique column design: Zero buffer retention and low elution volume (10 µl)
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
  • Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization
  • Unique column design: Zero buffer retention and low elution volume (10 µl)


Product Description


The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.

Technical Specifications

Applicable ForTransfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications.
Elution Volume≥ 10 µl
EquipmentMicrocentrifuge
Processing Time15 min
PurityTypical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0
Size RangeUp to ~200 kb
YieldUp to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture.

Resources


Q1: I accidently left my P3 Buffer at room temperature, is it still okay to use?

Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.

 

주문정보

CAT.No 품명 규격 비고
D4048 ZR BAC DNA Miniprep Kit 25 preps. Format: Spin-Column Sample Volume: 0.5 - 5.0 ml Processing Time: 15 minutes Elution Volume: ≥ 10 μl DNA Yield: ≤ 10 μg DNA Size Limits: 50 bp to ≥ 200 kb
D4049 ZR BAC DNA Miniprep Kit 100 preps. Format: Spin-Column Sample Volume: 0.5 - 5.0 ml Processing Time: 15 minutes Elution Volume: ≥ 10 μl DNA Yield: ≤ 10 μg DNA Size Limits: 50 bp to ≥ 200 kb