Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
The IFN-alpha is one of the member of the type I interferons (IFN)s family and it is mainly involved in innate immunity. IFN-alpha 1 is secreted by immune (lymphocytes, NK cells, B-cells and T-cells, macrophages) and non-immune cells (fibroblasts, endothelial cells, osteoblasts and others) in answer to a viral infection.
The main function of the IFN-alpha 1 is to alert the organism in case of viral infection by detection of abnormal double stranded DNA, but also to inhibit virus multiplication by action on the translation in infected cells.
An abnormal production of IFN-alpha 1 induces immune dysfunction such as autoimmune diseases (systemic lupus erythematosus, rheumatoid arthritis...) or mediates tissue inflammation.
Size: 96 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 6 months maximum after production
Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume: 100 µL
Tracer: horseradish peroxidase HRP
Detection Limit: ≤0,08 ng/mL
Standard Curve Range: 0,08-10,00 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104
Lengyel P. (1982).Biochemistry of interferons and their actions. Annual Review of Biochemistry. 51: 251–82
Pestka S., Langer J.A., Zoon K.C. et al. Interferons and their actions. 1987 Annual Review of Biochemistry. 56: 727–77
