Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Fast: Efficient cDNA synthesis from up to 5 µg RNA in less than 15 minutes
Simple Setup: Ready-to-use Master Mix. Just add RNA and incubate in a thermal cycler
Convenient: Room temperature reaction set-up
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Fast: Efficient cDNA synthesis from up to 5 µg RNA in less than 15 minutes
Simple Setup: Ready-to-use Master Mix. Just add RNA and incubate in a thermal cycler
Convenient: Room temperature reaction set-up
Product Description
ZymoScript RT PreMix Kit is an optimized reverse transcription kit containing all the necessary components for quick and efficient cDNA synthesis. Reaction setup can be completed at room temperature in less than one minute: simply add RNA to the ready-to-use RT PreMix. The RT PreMix includes a blue dye for easy tracking of pipetting, making reaction setup easier than ever. This dye ensures accurate reaction preparation and does not interfere with downstream analyses, including fluorescent detection methods.
The reverse transcription mix includes random hexamer and oligo dT primers, dNTPs, RNase inhibitors, reverse transcriptase, and an optimized reaction buffer for efficient, unbiased cDNA synthesis from as little as 0.1 pg to up to 5 µg of input RNA. Robust reverse transcription is achieved even with challenging templates that are GC-rich or contain secondary structures.ZymoScript RT PreMix is the kit of choice for studying the expression of a high number of targets from a single sample using 2-step RT-qPCR. For fast reverse transcription and robust qPCR amplification in a single tube, see ZymoScript One-Step RT-qPCR Kit (R3014).
Zymo Research is ready to support your RNA studies and help simplify your workflow. Please contacttech@zymoresearch.comfor more information.
Technical Specifications
Equipment
thermal cycler
Ideal Uses
Gene expression studies for many targets via 2-step RT-qPCR
The amount of total RNA required may vary depending on the expression level of the target transcripts. In general, we recommend using 0.1 pg - 5 µg of input RNA.
The dye used in ZymoScript RT PreMix has been thoroughly tested in downstream analyses. At the provided concentration, it will not affect fluorescent signal readings of any qPCR fluorescent channel.
Lower cDNA yields can be expected with degraded and/or impure RNA samples. We recommend using Quick-RNA or Direct-zol Kits (see Related Products) for high quality RNA extraction.
Yes, gene specific primers can be used to increase specific target yield. However, we recommend using ZymoScript One Step RT-qPCR (see Related Products) for enhanced specificity.
Samples can be treated with DNase I to eliminate DNA contamination. DNase I is included in the Quick-RNA and Direct-zol Kits recommended for RNA extraction (see Related Products). Alternatively, the DNase I set can be purchased separately.
No. The ZymoScript RT PreMix (2X) already contains a mixture of oligo dT and random hexamer primers which is not ideal for the synthesis of long cDNA molecules. For this specific application we recommend using the ZymoScript One-Step RT-qPCR Kit (R3014) with gene-specific primers.
