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DNA Clean & Concentrator-5

단2분만에 Carryover 없이 최소 볼륨 6μl로 DNA Clean-up 및 농축!

• Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution volume in as little as two
minutes with 0 μl wash residue carryover.
• Column and deep-well filtration plate designs allow DNA to be eluted at high
concentrations into minimal volumes of water or TE buffer.
• Eluted DNA is optimal for any down stream molecular biology application.

서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

HIGHLIGHTS

Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

DESCRIPTION

The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.

Detergent Tolerance	≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Elution Volume	≥ 6 µl
Equipment	Microcentrifuge
Purity	A260/A280 > 1.8
Sample Source	DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
Size Range	50 bp to 23 kb
Yield	≤ 5 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.

Q1: What is the difference between capped and uncapped?

The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.

Q2: What is the minimum input volume of DNA sample?

Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.

Q3: How many times can columns be reloaded?

Zymo Research recommends reloading columns no more than 5 times, as binding efficiency might decrease.

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

Q5: How can I process naked DNA stored in DNA/RNA Shield?

Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.

Q6: What is the lower limit and minimal amount of DNA that can be recovered?

Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

Q7: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?

The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

주문정보

CAT.No	품명	규격	비고
D4003	DNA Clean & Concentrator® -5 (uncapped columns)	50 preps.	Format: Spin-Column Elution Volume: ≥ 6 μl Processing Time: 2 minutes Binding Capacity: 5 μg DNA Size Limits: 50 bp - 23 kb
D4003T	DNA Clean & Concentrator® -5 (uncapped columns)	10 preps.	Format: Spin-Column Elution Volume: ≥ 6 μl Processing Time: 2 minutes Binding Capacity: 5 μg DNA Size Limits: 50 bp - 23 kb
D4004	DNA Clean & Concentrator® -5 (uncapped columns)	200 preps.	Format: Spin-Column Elution Volume: ≥ 6 μl Processing Time: 2 minutes Binding Capacity: 5 μg DNA Size Limits: 50 bp - 23 kb
D4013	DNA Clean & Concentrator® -5 (capped columns)	50 preps.	Format: Spin-Column Elution Volume: ≥ 6 μl Processing Time: 2 minutes Binding Capacity: 5 μg DNA Size Limits: 50 bp - 23 kb
D4014	DNA Clean & Concentrator® -5 (capped columns)	200 preps.	Format: Spin-Column Elution Volume: ≥ 6 μl Processing Time: 2 minutes Binding Capacity: 5 μg DNA Size Limits: 50 bp - 23 kb