Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q2: How can I process naked DNA stored in DNA/RNA Shield?
Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size).
Q3: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q5: How many times can columns be reloaded?
Zymo Research recommends no more than 5 times, as binding efficiency might decrease.
Q6: What is the minimum input volume of DNA sample?
Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommends raising the starting volume to 100 µl with water to ensure optimal binding conditions.
