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DNA/RNA Shield Lysis & Collection Tubes
Product Description

DNA/RNA Shield Lysis and Collection tubes are microbial lysis tubes and bead beating tubes designed for the streamlined collection, transport, and processing of biological specimen. The microbial lysis tubes and bead beating tubes are pre-filled with DNA/RNA Shield and a lysis matrix tailored for tough-to-lyse- sample inputs from tissue to microbial samples. Collected samples do not require additional handling for processing and are ready for homogenization. Bead sizes include 0.5mm & 0.1mm (R1103 and R1104) and 2.0mm (R1105).
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개



 

Product Description


DNA/RNA Shield Lysis and Collection tubes are microbial lysis tubes and bead beating tubes designed for the streamlined collection, transport, and processing of biological specimen. The microbial lysis tubes and bead beating tubes are pre-filled with DNA/RNA Shield and a lysis matrix tailored for tough-to-lyse- sample inputs from tissue to microbial samples. Collected samples do not require additional handling for processing and are ready for homogenization. Bead sizes include 0.5mm & 0.1mm (R1103 and R1104) and 2.0mm (R1105).

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Technical Specifications

Applicable ForNext-Generation Sequencing, qPCR, microarray
Device Specs2 mL screwcap tube pre-filled with DNA/RNA Shield (1 mL) and ultra-high density BashingBeads (R1103 - 0.5 mm & 0.1 mm beads, R1105 - 2.0 mm beads) for homogenization. R1104 includes swab with 20 mm breakpoint.
Device StorageAmbient temperature => 2 years
Sample Collection10% (v/v or w/v)
Sample SourceCells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc.
StabilityRNA: Ambient temperature
(4°C- 25°C) at least 1 month
DNA: Ambient temperature
(4°C- 25°C) at least 2 years
DNA & RNA: Frozen
(< -20°C): Indefinitely

Resources


Q1: What sample types is DNA/RNA Shield suitable for?

DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).

Q2: Can DNA/RNA Shield be used on extracted DNA and/or RNA?

Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).

Q3: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?

RNA > 1 month
DNA > 2 years

Q4: How long can samples be stored frozen in DNA/RNA Shield?

DNA & RNA (<-20°C): Indefinitely

Q5: Can I FACS sort directly into DNA/RNA Shield?

Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).

Q6: Can I still use DNA/RNA Shield if a precipitate has formed?

This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

Q7: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?

No, tissues do not need to be homogenized.

Q8: How to collect solid tissues in DNA/RNA Shield?

Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.

Q9: Is DNA/RNA ShieldTM compatible with bleach (sodium hypochlorite)?

Avoid mixing the reagent and bleach (sodium hypochlorite) or other strong oxidizing agents and acids. Clean up any accidental spills with water first.

Q10: Can the swab be submerged prior to collecting?

For any biological sample collection (nasal, throat, etc.), use a sterile swab for collection and then deposit it into DNA/RNA Shield. For application-specific questions, please reach out to technical support.

주문정보

CAT.No 품명 규격 비고
R1102 DNA/RNA Shield Collection Tube 50 pack
R1103 DNA/RNA Shield Lysis Tubes (Microbe) 50 pack
R1104 DNA/RNA Shield Lysis Tubes w/Swab (Microbe) 50 pack
R1105 DNA/RNA Shield Lysis Tubes (Tissue) 50 pack